Genetic diversity using single nucleotide polymorphisms (SNPs) and screening for salinity tolerance in rice germplasm at reproductive stage Profuse Microtillering of Androgenic Plantlets of Elite Indica Rice Variety IR 72 Conclusion: This scientific information could be used for solution of suitable parents, development of salt tolerant rice varieties, gene identification for salt tolerance and genetic diversity analysis.Ĭited References Fulltext Similar Articles It is confirmed that Holde Gotal, Bazra Muri and Hamai were salt tolerant compared to F元78. Unweighted pair group method of arithmetic means (UPGMA) dendrogram constructed from Neis (1972) genetic distance produced five distinct clusters of 25 rice genotypes. The average gene diversity of overall SSR loci for the 25 genotypes was 0.8885, ranged from 0.9024-0.8672. Results: The number of alleles/locus ranged from 10-12, with an average number of alleles of 11/locus and PIC values ranged from a low of 0.8533 (RM336) to a high of 0.8940 (RM585). Data were analyzed by POPGENE (version 1.31), Power Marker (version 3.25) and NTSYS-PC (version 2.2). These germplasms were characterized by 3 SSR markers which are RM510, RM585 and RM336. Among them, 25 germplasms were used for molecular study, which carry all tolerant variety found in phenotypic study (Hogla, Jamai Naru, Dakhsail, Patnai, Kute Patnai, Holde Gotal, Bazra Muri, Ghunshi, Tal Mugur, Nona Bokhra, Kashrail and F元78), 7 were moderately tolerant, 5 were highly susceptible and 1 was susceptible. Among them, 12 were found as salt tolerant, 13 were found as moderately tolerant, 29 were highly susceptible and 26 were susceptible by phenotypic analysis. Initially 80 germplasms were used to evaluate the salinity tolerance at seedling stage at glass-house following IRRI standard protocol. Materials and Methods: The study was conducted under Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh. The main objective of this study was to develop salt-tolerant rice varieties by identifying suitable parents and genetic diversity analysis. The conventional methods of plant selection for salt tolerance are difficult because of the large effects of the environment. The saline area is 3 times larger than land used for agriculture. Screening of Rice Landraces of Coastal Areas for Salt Tolerance at Seedling Stage Using Molecular Markersīackground and Objective: Salinity is becoming a serious problem in the world and a widespread soil problem in rice growing countries.
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